For large-scale cell culture, centrifugation is one method used to harvest the production cell culture.
Before you can purify your active pharmaceutical ingredient, you need to get rid of the cells (yes, after all that work secreting the API, we get rid of the cells).
Note: some APIs are not secreted, in which case you want to keep the cells, so harvest means different things in different processes.
To separate the cells from the cell culture fluid (CCF), you can filter it or centrifugate it. If you need to process 12,000L or 15,000L in hours, you're doing tangential flow filtration (TFF) or you're using a centrifuge.
If you're using an industrial centrifuge to clarify cell culture, chances are, you're using Alfa Laval.
Three minutes of your time is all it takes to see how large-scale centrifugation works.
Nice work,Alfa Laval, Westfalia
Before you can purify your active pharmaceutical ingredient, you need to get rid of the cells (yes, after all that work secreting the API, we get rid of the cells).
Note: some APIs are not secreted, in which case you want to keep the cells, so harvest means different things in different processes.
To separate the cells from the cell culture fluid (CCF), you can filter it or centrifugate it. If you need to process 12,000L or 15,000L in hours, you're doing tangential flow filtration (TFF) or you're using a centrifuge.
If you're using an industrial centrifuge to clarify cell culture, chances are, you're using Alfa Laval.
Three minutes of your time is all it takes to see how large-scale centrifugation works.
Nice work,
2 comments:
This is NOT an Alfa Laval machine, this is a GEA WESTFALIA machine. Watch the video till the end and you will see. And also, this is a "green" machine, most of the AL are blue.
noted.
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